Proteins play an important role in all cellular life. They can have structural functions, act as transporter, fulfil enzymatic roles or support signalling. To keep up with all these tasks, proteins need to be modified into different proteoforms, for example by adding glycans. Understanding this glycosylation process is of great interest for various research fields and for the pharmaceutical industry. One way to study these modifications is by mass spectrometry where glycosylation of proteins can be analysed after releasing glycans from the protein, digesting the protein into smaller peptides and glycopeptides or measuring the intact glycoprotein and deducing different proteoforms.
The following calculators help to predict MS spectra for all three cases: